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Open Access Review

Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors

by Shriya Madan a orcid  and  Yongzhi Chen b,* orcid
Department of Microbiology and Immunology, Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, USA
Department of Medicine, University of Massachusetts Chan Medical School, Worcester, USA
Author to whom correspondence should be addressed.
BAB  2023, 11; 2(2), 11;
Received: 27 September 2023 / Accepted: 16 October 2023 / Published Online: 17 October 2023


Pseudomonas aeruginosa is a common pathogen, and its presence in medical environments and water bodies has attracted widespread attention. Traditional detection methods are usually time-consuming and cumbersome, so it is necessary to develop a rapid and sensitive detection technology. DNase can specifically recognize and cut DNA molecules complementary to its substrate sequence. The researchers took advantage of this property to design various DNase-based sensors for detecting the presence of Pseudomonas aeruginosa. These sensors usually use DNase as a recognition element to identify target strains by hybridizing with specific DNA sequences. When the target strain is present, DNase is activated and begins to catalyze the cleavage reaction, producing a detectable signal. This DNase-based sensor has the advantages of rapidity, high sensitivity, and high specificity. In addition, the researchers also explored combining DNase with nanomaterials, fluorescent dyes, etc. to further improve the performance of the sensor. These improvements have improved the detection ability of the sensor in complex samples, laying the foundation for practical applications. With the continuous improvement of technology, these sensors are expected to be widely used in medical, environmental monitoring and other fields, and provide more efficient and convenient solutions for bacterial detection. This study reviewed the research progress of DNase-based sensors for the rapid detection of Pseudomonas aeruginosa.

Copyright: © 2023 by Madan and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (Creative Commons Attribution 4.0 International License). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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ACS Style
Madan, S.; Chen, Y. Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors. Biomaterials and Biosensors, 2023, 2, 11.
AMA Style
Madan S, Chen Y. Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors. Biomaterials and Biosensors; 2023, 2(2):11.
Chicago/Turabian Style
Madan, Shriya; Chen, Yongzhi 2023. "Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors" Biomaterials and Biosensors 2, no.2:11.
APA style
Madan, S., & Chen, Y. (2023). Advances in rapid detection of Pseudomonas aeruginosa with DNase-based sensors. Biomaterials and Biosensors, 2(2), 11.

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