In the past, researchers investigated the immune response acting against mycobacterial antigens in tuberculosis (TB). Several ELISA tests have been conducted for the diagnosis of tuberculosis. Other researchers manipulated 16 kDa antigen from Mycobacterium Tuberculosis to develop a tuberculosis diagnostic test while some others studied the subspecies of M. tuberculosis. In the present study, we explored the possibilities of detecting 16 kDa protein derived from M. tuberculosis, on an interdigitated electrode (IDE). Measurements with Electrochemical impedance spectroscopy and current-volt systems were demonstrated using antibody as the probe. Further, the involvement of gold nanoparticle for the signal-enhancement and high-performance are evidenced. The specificity in the serum containing samples is used to show the clinical relevancy. 16 kDa antigen used in this experiment is highly reliable for TB diagnosis as it largely expresses and can be implemented in other sensing system.
Rani, S.; Gopinath, S. C. Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors, 2022, 1, 1. https://doi.org/10.58567/bab01010001
Rani S, Gopinath S C. Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors; 2022, 1(1):1. https://doi.org/10.58567/bab01010001
Rani, S.S.; Gopinath, Subash C. 2022. "Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode" Biomaterials and Biosensors 1, no.1: 1. https://doi.org/10.58567/bab01010001
Rani, S., & Gopinath, S. C. (2022). Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors, 1(1), 1. https://doi.org/10.58567/bab01010001
Article Access Statistics
Y. Qu, G. Zoppi, R.W. Miles, A novel quinternary alloy ( Cu2Zn 1 − x Cd x SnS 4 ) nanostructured sensor for biomedical diagnosis, Mater. Res. Express. 3 (n.d.) 1–8. https://doi.org/10.1088/2053-1591/3/8/085022.
M.C. Rodr??guez, G.A. Rivas, Label-free electrochemical aptasensor for the detection of lysozyme, Talanta. 78 (2009) 212–216. https://doi.org/10.1016/j.talanta.2008.11.002.
J.H. An, S.J. Park, O.S. Kwon, J. Bae, J. Jang, High-performance flexible graphene aptasensor for mercury detection in mussels, ACS Nano. 7 (2013) 10563–10571. https://doi.org/10.1021/nn402702w.
J.B. Russell, F.F. Syed, M. Ntsekhe, B.M. Mayosi, S. Moosa, M. Tshifularo, J.P. Smedema, Tuberculous effusive-constrictive pericarditis., Cardiovasc. J. Afr. 19 (2008) 200–201.
B. Samten, X. Wang, P.F. Barnes, Mycobacterium tuberculosis ESX-1 system-secreted protein ESAT-6 but not CFP10 inhibits human T-cell immune responses, Tuberculosis. 89 (2009). https://doi.org/10.1016/S1472-9792(09)70017-4.
T. Lakshmipriya, S.C.B. Gopinath, U. Hashim, V. Murugaiyah, Multi-analyte validation in heterogeneous solution by ELISA, Int. J. Biol. Macromol. 105 (2017) 796–800. https://doi.org/10.1016/j.ijbiomac.2017.07.115.
C. Toyos-Rodríguez, F.J. García-Alonso, A. de la Escosura-Muñiz, Electrochemical biosensors based on nanomaterials for early detection of alzheimer’s disease, Sensors (Switzerland). 20 (2020) 1–43. https://doi.org/10.3390/s20174748.
A.H.M.S. Yusoff MN Jamlos, MF, Synthesis of Superparamagnetic Hydroxyapatite Core-Shell Nanostructure by a Rapid Sol-Gel Route, E-Journal Surf. Sci. Nanotechnol. 15 (2017) 121–126. https://doi.org/10.1380/ejssnt.2017.121.
M. Soto-ribeiro, B. Wehrle-haller, L. Cognet, B. Lounis, A Highly Speci fi c Gold Nanoprobe for Live-Cell Single-Molecule Imaging, (2013) 1–14.
Z. Xiu, Q. Zhang, H.L. Puppala, V.L. Colvin, P.J.J. Alvarez, Negligible particle-specific antibacterial activity of silver nanoparticles., Nano Lett. 12 (2012) 4271–5. https://doi.org/10.1021/nl301934w.
Y. Yang, A.M. Asiri, Z. Tang, D. Du, Y. Lin, Graphene based materials for biomedical applications, Mater. Today. 16 (2013) 365–373. https://doi.org/10.1016/j.mattod.2013.09.004.
S.G. Meirinho, L.G. Dias, A.M. Peres, L.R. Rodrigues, Electrochemical aptasensor for human osteopontin detection using a DNA aptamer selected by SELEX, Anal. Chim. Acta. 987 (2017) 25–37. https://doi.org/10.1016/j.aca.2017.07.071.
H. Kim, J.U. Lee, S. Kim, S. Song, S.J. Sim, A Nanoplasmonic Biosensor for Ultrasensitive Detection of Alzheimer’s Disease Biomarker Using a Chaotropic Agent, ACS Sensors. 4 (2019) 595–602. https://doi.org/10.1021/acssensors.8b01242.
E. Tu, P. Pearlmutter, M. Tiangco, G. Derose, L. Begdache, A. Koh, Comparison of Colorimetric Analyses to Determine Cortisol in Human Sweat, ACS Omega. (2020) 8211–8218. https://doi.org/10.1021/acsomega.0c00498.
T. Lakshmipriya, Y. Horiguchi, Y. Nagasaki, Co-immobilized poly(ethylene glycol)-block-polyamines promote sensitivity and restrict biofouling on gold sensor surface for detecting factor IX in human plasma, Analyst. 139 (2014) 3977–3985. https://doi.org/10.1039/c4an00168k.
J. Zhang, T. Lakshmipriya, S.C.B. Gopinath, Electroanalysis on an Interdigitated Electrode for High-Affinity Cardiac Troponin i Biomarker Detection by Aptamer-Gold Conjugates, ACS Omega. 5 (2020) 25899–25905. https://doi.org/10.1021/acsomega.0c03260.
J. Zhao, W. Chang, L. Liu, X. Xing, C. Zhang, H. Meng, S.C.B. Gopinath, T. Lakshmipriya, Y. Chen, Y. Liu, Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay, J. Immunol. Methods. 489 (2021) 112942. https://doi.org/10.1016/j.jim.2020.112942.
C. Ibau, M.K.M. Arshad, S.C.B. Gopinath, M. Nuzaihan, M.F.M. Fathil, S.A. Shamsuddin, Immunosensing prostate-specific antigen: Faradaic vs non-Faradaic electrochemical impedance spectroscopy analysis on interdigitated microelectrode device, Int. J. Biol. Macromol. 162 (2020) 1924–1936. https://doi.org/10.1016/j.ijbiomac.2020.08.125.
Y. Wang, Y. Guo, J. Lu, Y. Sun, X. Yu, S.C.B. Gopinath, T. Lakshmipriya, Y.S. Wu, C. Wang, Nanodetection of Head and Neck Cancer on Titanium Oxide Sensing Surface, Nanoscale Res. Lett. 15 (2020) 33. https://doi.org/10.1186/s11671-020-3262-x.
S.K. Srivastava, V.J.B. Ruigrok, N.J. Thompson, A.K. Trilling, A.J.R. Heck, C. van Rijn, J. Beekwilder, M.A. Jongsma, 16 kDa Heat Shock Protein from Heat-Inactivated Mycobacterium tuberculosis Is a Homodimer - Suitability for Diagnostic Applications with Specific Llama VHH Monoclonals, PLoS One. 8 (2013). https://doi.org/10.1371/journal.pone.0064040.